FastQCFastQC Report
Thu 26 May 2016
ERR488973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22590713
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412190.1824599338675145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282590.12509122664698544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1600600.025.9827181
GGTATCA1000900.025.7401921
TACCTGG221000.021.7762992
GAGTACT1006200.021.62094512
TATCAAC1923700.021.4785022
AGTACTT1028250.021.1888913
ATCAACG1963850.021.005723
TCAACGC1976050.020.8708024
GTACCTG239300.020.6796471
CAACGCA2002800.020.5739025
GTACATG1621250.020.4493241
GTACTTT1064450.020.25309814
AACGCAG2053800.020.124756
TACATGG1633600.019.9827562
AGAGTAC1950400.019.93181611
ACATGGG1650150.019.195993
TACTTTT1179200.018.30245215
ACTTTTT1209850.018.03553816
ACGCAGA2300350.017.9199717
CATGGGG779050.017.783724
Produced by FastQC (version 0.11.5)