FastQCFastQC Report
Thu 26 May 2016
ERR488973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22590713
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388500.17197332372820637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286670.12689727854096505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA989250.026.215321
GTATCAA1555350.025.2288361
TACCTGG221750.020.8472212
TATCAAC1887450.020.6634142
GAGTACT1015200.020.48728212
AGTACTT1035750.020.18449813
ATCAACG1931250.020.1376573
TCAACGC1941550.020.0467744
GTACCTG238800.019.8402521
CAACGCA1970850.019.740085
GTACATG1630150.019.3121971
AACGCAG2022700.019.2694956
GTACTTT1073450.019.25707214
TACATGG1644600.018.864762
AGAGTAC1984950.018.70071211
ACATGGG1648500.018.2174593
TACTTTT1187000.017.4436715
ACTTTTT1208100.017.27036516
ACGCAGA2279950.017.0826957
CGCAGAG2305150.016.9067218
Produced by FastQC (version 0.11.5)