FastQCFastQC Report
Thu 26 May 2016
ERR488972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477092
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217000.1498919810691263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157600.10886164155066501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA418750.027.2621461
GTATCAA670250.026.4070761
GTACCTG164450.021.748891
TACCTGG161900.021.5669562
TCAACGC813400.021.5129974
ATCAACG814850.021.4827653
TATCAAC822600.021.3750972
CAACGCA827100.021.116975
AACGCAG856150.020.4969026
GAGTACT499900.020.44040712
AGTACTT510950.019.98653413
AGAGTAC845350.019.66847211
GTACTTT532400.019.1219414
GTACATG592700.018.3722571
ACCTGGG183350.018.3241313
ACGCAGA962250.018.1884127
CGCAGAG976450.017.9149428
TACTTTT572550.017.78760315
TACATGG610350.017.5483282
ACTTTTT587500.017.46122616
Produced by FastQC (version 0.11.5)