FastQCFastQC Report
Thu 26 May 2016
ERR488972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477092
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217150.14999559303760726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170640.11786897534394337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA453850.026.1945021
GTATCAA706200.025.1706161
TACCTGG160950.020.8375762
GTACCTG165500.020.7745361
GAGTACT528650.020.60844812
ATCAACG860300.020.418563
TCAACGC860750.020.4119634
TATCAAC870200.020.293322
CAACGCA876350.020.0525465
AGTACTT542050.020.01624913
AACGCAG905650.019.4838036
GTACTTT561600.019.23960114
AGAGTAC879500.019.06738711
TATAACG11350.018.2401032
TACTTTT604150.017.85502615
ACTTTTT620200.017.54864116
ACCTGGG181550.017.3518753
GTACATG602000.017.3464091
ACGCAGA1020900.017.2400157
CGCAGAG1035150.016.993578
Produced by FastQC (version 0.11.5)