FastQCFastQC Report
Thu 26 May 2016
ERR488971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19285416
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA754410.3911816058310591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT559700.020.41243712
AGTACTT564950.020.10156213
GTACTTT573450.019.73237214
AGAGTAC1104350.018.78843511
TACTTTT608750.018.5093915
GTACATG831150.018.1412451
ACTTTTT640850.017.94126916
TACATGG844400.017.5904482
ACATGGG874950.016.4817183
TACCTGG184450.016.0007842
GTACCTG197250.015.6664031
GTGTACG17650.015.2585461
CATGGGG554050.014.6031094
GTATAGG30700.014.5082361
GTCCTAC40800.014.4710231
CTTTTTT816500.014.19111117
GTATAAT34600.013.8708141
TCGACCG7300.013.69958118
GAGTACG30100.013.6503481
TGTACGC15550.013.3190932
Produced by FastQC (version 0.11.5)