FastQCFastQC Report
Thu 26 May 2016
ERR488971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19285416
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2327411.2068238507274098No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA737460.38239258100525286No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC403330.2091373087311158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT569000.020.15948712
AGTACTT577050.019.69905713
GTACTTT587950.019.26921314
TACTTTT620750.018.31155615
AGAGTAC1120250.018.30783511
GTACATG827100.017.811811
ACTTTTT647950.017.69193816
TACATGG847650.016.9745542
GAGTACG29050.016.3091341
ACATGGG871850.016.0476363
TACCTGG181500.015.9881522
GTACCTG196550.015.3954161
GTATAGG30100.015.2806431
GTGTAGC89400.014.2739891
GTAGGAC42650.014.1573193
CATGGGG551300.014.1005234
CTTTTTT818000.014.03484317
TCGACCG5950.013.91463618
TACGCAG28200.013.5811294
TATACTG47300.013.3487545
Produced by FastQC (version 0.11.5)