FastQCFastQC Report
Thu 26 May 2016
ERR488970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945048
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300810.21571098213502027No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA262400.1881671543905765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215280.15437738184909797No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199680.1431906150484387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161880.11608421856991816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG280850.037.8437422
GTACCTG288050.037.5355721
ACCTGGG305000.034.0373423
CCTGGGG247350.027.5056044
TACACGG25800.023.8542042
TATCACG9350.023.6664412
TATAACG13800.022.549042
GTACACG32450.021.6373121
GTATCAA814750.021.4849281
GGTATCA573600.020.7128371
GTACATG550000.020.0608791
TAACGCA16800.019.9604554
ATAACGC17050.019.6674233
GAGTACT528400.019.4767412
AGAGTAC831700.019.19582411
TACATGG566750.019.1315062
GTACCCG27350.019.0959631
AGTACTT537700.019.01307313
CTGGGGG145450.018.2957325
ACATGGG583550.018.0033283
Produced by FastQC (version 0.11.5)