FastQCFastQC Report
Thu 26 May 2016
ERR488970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945048
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277440.19895234494710953No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA261300.18737834391104283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215830.1547717870888648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168750.12101069856482387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166920.11969840476705423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG287150.037.6906972
GTACCTG294350.037.0489651
ACCTGGG310700.033.923333
CCTGGGG242350.028.3286614
TATAACG13950.025.9683042
TACACGG26100.024.1901782
TATCACG10250.022.5517082
ATAACGC18750.021.3444633
TAACGCA16700.021.2782944
GGTATCA576350.020.9150491
GTACCCG29400.020.1298471
GTATCAA799000.020.0421561
GTACACG34050.019.9218961
CTGGGGG138000.019.8245775
TACCCGG28700.019.7147122
GTATAAC25350.018.5674671
GTACATG552600.018.0123211
GAGTACT536750.017.56542612
AGAGTAC856550.017.38274211
AGTACTT541850.017.3246913
Produced by FastQC (version 0.11.5)