FastQCFastQC Report
Thu 26 May 2016
ERR488969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18371199
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226870.12349221191278806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT612750.021.24489412
AGTACTT630050.020.65709913
AGAGTAC996200.020.21702811
GTACTTT643550.020.04625914
GTACCTG159450.019.6576041
GTATCAA1209450.018.7861981
TACTTTT704650.018.29767815
TACCTGG165650.018.1998752
ACTTTTT735000.017.83835416
GTACATG671250.017.8102321
TACATGG674600.017.2751222
GTGTAGC101450.016.6100481
ACATGGG679850.016.5842953
TATCAAC1363850.016.505172
ATCAACG1364200.016.4483153
TCAACGC1370450.016.3812034
CAACGCA1385750.016.1777885
AACGCAG1415300.015.888096
TAGCCCT113850.015.4303544
GGTATCA892250.015.1009021
Produced by FastQC (version 0.11.5)