FastQCFastQC Report
Thu 26 May 2016
ERR488969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18371199
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227150.12364462439277915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT630000.020.38137412
AGTACTT651900.019.67551213
AGAGTAC1016500.019.32442311
GTACTTT660650.019.32092514
GTACCTG157950.019.0974181
GTATCAA1220750.017.8465251
TACTTTT733550.017.4000615
TACCTGG167600.017.3951742
ACTTTTT758600.016.84822316
GTACATG677450.016.6565271
TACATGG680300.016.244362
TATCAAC1384950.015.61745552
ACATGGG681250.015.5582533
GGTATCA915350.015.5321791
ATCAACG1385100.015.5036733
TAGCCCT121450.015.4542774
TCAACGC1389550.015.4490984
CAACGCA1406600.015.2544755
AACGCAG1431450.015.01857956
GTGTAGC110550.014.9257921
Produced by FastQC (version 0.11.5)