FastQCFastQC Report
Thu 26 May 2016
ERR488968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17611994
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187150.10626281158169824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG285150.030.4723172
GTACCTG298800.029.5158841
ACCTGGG316050.026.4852523
TATCACG10750.026.011912
GTATCAA1040650.021.4691621
GAGTACT541650.020.71704112
CCTGGGG249650.020.5887534
AGTACTT553850.020.3488613
GTACATG831750.020.2476881
AGAGTAC1032850.019.66185811
TACATGG855000.019.4009482
GTACTTT587100.019.00349814
TATAACG12650.018.8301682
GTACACG44300.018.4739671
ACATGGG873800.018.4126053
GGTATCA733900.018.2938391
TACACGG37850.017.9678462
TATCAAC1239150.017.9330962
TACTTTT622950.017.91543815
TCAACGC1244100.017.8494644
Produced by FastQC (version 0.11.5)