FastQCFastQC Report
Thu 26 May 2016
ERR488968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17611994
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184820.1049398495139165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG289600.030.5725522
GTACCTG306450.029.340091
TATAACG10050.028.4961132
ACCTGGG317600.026.9635053
GTATCAA1058550.021.0323261
CCTGGGG246200.020.951174
GAGTACT564050.019.98158312
AGTACTT572000.019.68599713
GTACATG837500.019.1799981
AGAGTAC1071650.019.15090811
TATCACG12750.018.9435542
GGTATCA749550.018.5654321
GTACTTT602850.018.51830914
TACATGG862500.018.3182982
TACACGG35150.017.5711962
ACATGGG876400.017.5112653
ATCAACG1261300.017.397783
TCAACGC1260900.017.395094
TATCAAC1265250.017.3905642
TACTTTT646050.017.2634715
Produced by FastQC (version 0.11.5)