FastQCFastQC Report
Thu 26 May 2016
ERR488967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21060917
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328650.034.3799861
GTATCAA605600.032.1488341
ATCAACG758150.025.2965833
TCAACGC758950.025.2938774
TATCAAC768850.025.1452242
CAACGCA776700.024.683535
AACGCAG813750.023.6792246
TACCTGG242600.023.2952272
GTACCTG251450.023.085651
ACGCAGA930400.020.6008477
CGCAGAG955900.020.0503798
GAGTACT495100.019.92587912
AGAGTAC923300.019.76706911
GTACATG782150.019.0774461
AGTACTT519100.019.02556413
TACATGG793250.018.5443062
ACCTGGG292550.018.5248363
GTACTTT528100.018.46687314
CAGAGTA1014850.018.43558510
GCAGAGT1034350.018.2077799
Produced by FastQC (version 0.11.5)