FastQCFastQC Report
Thu 26 May 2016
ERR488967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21060917
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA333100.034.7777371
GTATCAA603150.031.3841671
ATCAACG762450.024.3954773
TCAACGC761600.024.3728734
TATCAAC772600.024.165452
GTACCTG249100.023.8773351
CAACGCA779450.023.8232865
TACCTGG242300.023.724312
AACGCAG813650.022.9034716
ACGCAGA943750.019.7458027
GAGTACT523550.019.23412
CGCAGAG968050.019.2257928
ACCTGGG286850.019.1968253
GTACATG786500.019.015971
AGAGTAC952850.018.97506511
AGTACTT546250.018.49172413
TACATGG803050.018.2177722
GTACTTT551300.018.21595814
CAGAGTA1042000.017.76215410
GCAGAGT1061300.017.5722089
Produced by FastQC (version 0.11.5)