FastQCFastQC Report
Thu 26 May 2016
ERR488966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14227624
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA466930.3281855072920116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243960.1714692488359265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180800.12707673466771402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG180900.022.546133
GTACCTG208500.021.9013751
TACCTGG206300.021.4156442
GAGTACT466550.020.0598712
AGTACTT471700.019.68775413
GTACATG758950.019.2263531
TACATGG766250.018.826842
GTACTTT495900.018.63128314
TACCCGG18550.018.2634012
TAGCCCT92900.018.0066764
ACATGGG774150.017.9664133
AGAGTAC918650.017.80669611
GTGTAGC86550.017.227161
TATAACG8250.017.1802482
GGGTACC80900.017.1700467
TACTTTT546450.016.98812515
GGTACCT80850.016.930518
CATGGGT151250.016.8870164
GTATAGC61100.016.8158451
ACTTTTT565600.016.71637316
Produced by FastQC (version 0.11.5)