FastQCFastQC Report
Thu 26 May 2016
ERR488966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14227624
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241180.16951530346880125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195100.13712760472163166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG180200.020.6576273
GTACCTG202250.020.1269381
TACCCGG17950.019.987422
TACCTGG201450.019.7960452
GAGTACT478900.019.5771612
AGTACTT487100.019.18385913
TATAACG9650.018.9468232
GTACCCG20500.018.3891831
GTACTTT506100.018.3341614
GTACATG756650.017.9862021
TACATGG763950.017.529862
GTATAGG36450.017.1739771
AGAGTAC951950.017.08853111
TACTTTT550600.016.93984615
ACATGGG773800.016.8173183
GTATCAA1081600.016.7521881
ACTTTTT566950.016.49391616
TAGCCCT91450.016.2592774
GTGTAGC91100.015.9828241
CATGGGT153600.015.475184
Produced by FastQC (version 0.11.5)