FastQCFastQC Report
Thu 26 May 2016
ERR488965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18388782
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230960.12559831314548184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG356050.037.475162
GTACCTG362150.037.2657281
ACCTGGG369350.035.11063
CCTGGGG281550.027.682684
TATAACG17100.025.4707432
GTACACG39450.021.1236821
TACACGG36500.021.0245782
ATAACGC22150.020.9095463
GTACCCG30300.020.3131351
GTATCAA960250.020.0608081
GTACATG634900.019.5137631
GAGTACT571650.019.0565812
AGAGTAC909600.018.9710611
TACCCGG30150.018.9175032
TACATGG643600.018.884262
AGTACTT588900.018.5120613
TATCACG13650.018.4866142
TAACGCA24950.018.1475434
ACATGGG656700.017.8000053
CTGGGGG167800.017.7087425
Produced by FastQC (version 0.11.5)