FastQCFastQC Report
Thu 26 May 2016
ERR488965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18388782
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218310.11871911908031756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG350400.036.7973861
TACCTGG347700.036.624112
ACCTGGG362450.034.2769933
CCTGGGG272250.027.8154494
TATAACG18250.023.2525162
GTACCCG30900.022.6220051
TACACGG37650.021.2593862
TACCCGG32350.020.6897452
ATAACGC21700.020.6686133
GTACACG42900.020.1660561
GTATCAA956700.019.4998721
TAACGCA23200.019.3319134
GAGTACT579400.018.92228712
AGAGTAC940050.018.24625211
CTGGGGG155600.018.2254945
AGTACTT602100.018.1061513
GTACATG632450.017.8099631
TACATGG642950.017.2999762
TATCACG12750.016.7768042
GTACTTT649200.016.69687814
Produced by FastQC (version 0.11.5)