FastQCFastQC Report
Thu 26 May 2016
ERR488964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779832
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA334740.3105243198595303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152460.14143077554455394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG205950.040.1247521
TACCTGG204450.039.9827162
ACCTGGG225950.035.3561633
CCTGGGG170400.028.5069274
TATAACG10900.024.0999032
GTACCCG18300.024.0962721
ATAACGC11250.023.956963
TAACGCA11200.022.8269754
GTACACG23300.022.7998891
TACCCGG18300.022.6651382
TACACGG23300.021.3616492
TATCACG7450.021.3417362
TACCGGG12600.019.476732
GAGTACT355400.019.394912
GTACATG330950.019.325221
GTACCGG18000.018.9038731
AGTACTT366950.018.785413
AGAGTAC536150.018.74014911
TACATGG335100.018.5972822
CTGGGGG98950.018.119475
Produced by FastQC (version 0.11.5)