FastQCFastQC Report
Thu 26 May 2016
ERR488964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779832
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA358190.3322779056296981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151010.14008567109394654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113780.10554895475179947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG219050.040.4773031
TACCTGG217750.040.2464682
ACCTGGG232250.036.7829973
CCTGGGG166800.029.6605434
TAACGCA12350.027.3769554
ATAACGC13300.025.4229933
TATAACG13200.025.3542042
TACCCGG17850.025.1279662
TATCACG7200.023.960162
GTACCCG19850.023.3534181
GTACACG23300.023.1619361
TACACGG22800.021.3371522
CTGGGGG99450.018.6640345
GAGTACT360800.018.4630512
GTACATG330800.018.426661
TACCGGG12850.018.2582022
AGAGTAC549400.018.10907711
GTATAAC28100.018.0974431
TACATGG333600.017.7994692
AGTACTT375450.017.71514313
Produced by FastQC (version 0.11.5)