FastQCFastQC Report
Thu 26 May 2016
ERR488963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12991496
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197800.15225344332939023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150510.11585270857182267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA418950.026.1748491
GTATCAA649750.025.3901771
TACCTGG156150.022.9473842
GTACCTG160950.022.739491
TATCAAC793350.020.6640382
TCAACGC795250.020.5442524
ATCAACG797700.020.5101663
GAGTACT454150.020.22070312
CAACGCA808050.020.2104515
GTACATG599650.019.8303851
AGTACTT460850.019.77791813
AACGCAG829500.019.7361976
ACCTGGG174050.019.5737293
GTACTTT476100.019.08055714
TACATGG610700.019.0097662
AGAGTAC813050.018.75925811
TACTTTT500800.018.13602315
ACATGGG629000.017.905933
ACTTTTT528350.017.5458116
ACGCAGA931750.017.4968857
Produced by FastQC (version 0.11.5)