FastQCFastQC Report
Thu 26 May 2016
ERR488963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12991496
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199700.15371593848776155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160760.12374248508408886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA424650.026.5224291
GTATCAA656000.025.1654821
TACCTGG151750.022.350772
GTACCTG160100.021.7286781
TATCAAC805550.020.3669362
TCAACGC805100.020.257154
ATCAACG808450.020.1774793
GAGTACT476450.020.02028312
CAACGCA817450.019.946275
AGTACTT481250.019.73473213
AACGCAG839200.019.4598056
GTACTTT498350.019.07833714
ACCTGGG171950.018.9012853
GTACATG603600.018.8483491
AGAGTAC843550.018.61998211
TACATGG611100.018.2767122
TACTTTT522300.018.15684715
ACTTTTT542150.017.5875116
ACGCAGA947800.017.2118157
ACATGGG624050.017.122393
Produced by FastQC (version 0.11.5)