FastQCFastQC Report
Thu 26 May 2016
ERR488962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825687
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240220.20313407584692544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171920.1453784460894323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG209450.030.9317112
GTACCTG214250.030.6333541
ACCTGGG212500.029.1675243
GGTATCA393850.029.1138631
GTATCAA649050.027.2703111
CCTGGGG151200.023.9762944
TATAACG7000.023.2244972
TATCACG7250.021.9465522
TACACGG25100.021.7735822
TATCAAC813750.021.7590852
ATCAACG810350.021.7466353
TCAACGC814500.021.6511654
CAACGCA827700.021.3374465
GTACACG28950.021.2313821
GAGTACT479000.020.9238112
AACGCAG852550.020.7881416
AGTACTT485950.020.4765313
GTACATG679650.020.1667271
TACATGG687500.019.5563762
AGAGTAC881650.019.2552611
Produced by FastQC (version 0.11.5)