FastQCFastQC Report
Thu 26 May 2016
ERR488962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825687
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225840.1909741057749964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165730.14014407788739885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG210100.030.3800352
GTACCTG214150.030.3493291
GGTATCA384700.029.095561
ACCTGGG215850.028.483823
GTATCAA626000.027.058031
TATAACG7500.026.2214182
CCTGGGG158650.022.5082154
ATCAACG789300.021.340193
TCAACGC792800.021.2850554
GTACCCG20250.021.180541
TATCAAC799100.021.1777822
CAACGCA807700.020.9095755
TACCCGG19700.020.8412282
AACGCAG831700.020.3891646
GAGTACT471100.020.30685212
AGTACTT483450.019.7738313
GTACATG680150.019.757261
TACATGG691950.019.117012
ACATGGG693600.018.5094363
GTACTTT513300.018.48950214
Produced by FastQC (version 0.11.5)