FastQCFastQC Report
Thu 26 May 2016
ERR488961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16629679
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220990.1328889150536219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA366750.030.2166271
GTATCAA609450.029.0674231
GTACCTG225650.028.1646961
TACCTGG222900.027.892682
ACCTGGG263850.022.8401183
TATCAAC771300.022.8321422
TCAACGC775200.022.6810194
ATCAACG780800.022.5077043
CAACGCA786550.022.326565
AACGCAG823800.021.473266
GAGTACT484150.020.28167212
AGTACTT498250.019.74995813
GTACATG692800.019.469761
AGAGTAC880400.019.21621511
ACGCAGA933800.018.8771927
GTACTTT519850.018.71709614
TACATGG713450.018.5926272
CGCAGAG958450.018.3712318
ACATGGG736650.017.5543773
TACTTTT559950.017.43930415
Produced by FastQC (version 0.11.5)