FastQCFastQC Report
Thu 26 May 2016
ERR488961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16629679
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219660.13208914014515855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA372200.028.9319841
GTATCAA608250.028.3674181
GTACCTG231050.027.5777681
TACCTGG232300.026.8098582
TATCAAC771150.022.2955682
TCAACGC768900.022.2480224
ATCAACG774350.022.0608463
ACCTGGG273800.021.9019533
CAACGCA783050.021.8680235
AACGCAG817850.021.059876
TATAACG9800.020.7726762
GAGTACT491250.020.07752812
GTACATG678900.019.4232791
AGTACTT507100.019.39555513
AGAGTAC884150.018.7484811
GTACTTT523100.018.64402614
ACGCAGA933000.018.4097277
TACATGG706300.018.407222
CGCAGAG957300.017.9467778
ACATGGG724400.017.509093
Produced by FastQC (version 0.11.5)