FastQCFastQC Report
Thu 26 May 2016
ERR488960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10301462
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9654339.371805671855121No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2941332.8552549143024555No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG2052351.9922900264059609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414650.4025156817546869No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389620.37821815971364064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310000.300928159517552No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA231580.22480304251959576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208000.20191308767629293No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAA167690.16278271957902674No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151460.14702767432428523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151100.1466782093648455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106840.10371343407372662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG63800.039.0693471
CTTATGT75200.032.9917032
TATGTAC76400.032.242454
TTATGTA76350.032.2192963
GAGTACG71450.030.3253421
TACCTGG45900.029.8190632
AGTACGC74400.028.9219952
TACGCAG77000.027.8966964
GTACGCA77950.027.5132123
GTACCTG51000.027.1905351
CTTAACG1555.2750693E-1126.68704621
CGCAGTG80900.026.4093596
ACGCAGT83350.025.6853495
ACCTGGG50500.025.659433
CTTTGCG21200.023.8630852
GTACACG6200.023.4847281
GTACCGG3450.023.1119541
ATGCGAC2250.022.9805121
TATAACG4400.022.8378332
TAGGCAG36750.022.2487934
Produced by FastQC (version 0.11.5)