FastQCFastQC Report
Thu 26 May 2016
ERR488959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19002844
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210290.11066238295699318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG250800.029.5998671
TACCTGG248950.029.2904052
ACCTGGG288250.024.3572483
TATAACG13050.020.9801372
GAGTACT532650.019.4949412
AGTACTT546800.018.92911313
GTACATG684100.018.7079011
CCTGGGG239650.018.6831024
TACACGG38750.018.513582
AGAGTAC915050.018.49878711
TACATGG693250.018.1171572
GTACTTT579650.017.82562614
ATAACGC17150.017.7818373
GTACACG44950.017.4332891
ACATGGG715100.016.9903853
TATCACG14700.016.9748882
TACTTTT618150.016.77100215
ACTTTTT662300.016.11716516
GAGTACG27550.015.9838991
TAACGCA20250.015.9155574
Produced by FastQC (version 0.11.5)