FastQCFastQC Report
Thu 26 May 2016
ERR488959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19002844
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1106300.5821760153374937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206100.1084574498427709No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA196740.1035318713346276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG255400.030.5258371
TACCTGG256900.029.613652
ACCTGGG297150.024.8128263
TATCACG13650.019.9683382
GAGTACT553050.019.38709312
CCTGGGG248000.019.0180764
AGTACTT569450.018.74393513
TATAACG11900.018.5558342
AGAGTAC945500.018.30897511
GTACATG696950.017.7025591
GTACTTT607500.017.55289614
GTACACG42850.017.5163461
TACATGG703900.017.258482
TACTTTT640600.016.6400215
ACATGGG721200.016.1651573
TACACGG37400.015.9595992
ACTTTTT675150.015.89574716
GAGTACG27350.015.0495751
ATAACGC16750.014.8308193
CATGGGG449100.014.2665374
Produced by FastQC (version 0.11.5)