FastQCFastQC Report
Thu 26 May 2016
ERR488958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453364
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384050.36739369259503446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289650.2770878350739532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239840.2294381024137302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200950.19223476767861522No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG121290.11602963409673671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA11550.020.91473865
GAGTACT487600.020.90760612
AGTACTT495900.020.59352513
GTACTTT520450.019.47561814
TACCTGG97300.018.9380782
CATGGGT84350.018.9283564
GTACATG820850.018.8918741
GTACCTG98550.018.847511
GCTCGAT13300.018.68173864
TACATGG831200.018.5434932
ACATGGG824200.018.1482623
ACTTTTT569350.017.96352416
CGTACGA7700.017.9245860
TACTTTT566000.017.92023515
CGTCATT13950.017.79534526
CATGGGG356450.017.5864034
TCGATAT3750.016.56383161
AGAGTAC1074050.016.36401711
GTACGTA7800.015.91191611
CATGGGC183350.015.5081264
Produced by FastQC (version 0.11.5)