FastQCFastQC Report
Thu 26 May 2016
ERR488958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453364
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389510.37261689155758854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311150.29765537677631815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218820.20932974303774365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215660.20630679272241934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105110.10055136317839884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT519450.020.1633412
AGTACTT527950.019.91712413
TACCTGG95050.019.7469042
GTACCTG99900.019.1774461
GTACTTT549400.019.00763914
GTACATG841200.018.0185011
TCGATAT4600.018.00275261
GCTCGAT12900.017.9220364
CATGGGT84800.017.7803334
TACATGG849800.017.6654452
ACTTTTT599050.017.57522816
TACTTTT600050.017.45970315
GCGCGTA3400.017.24941813
ACATGGG837700.017.2121893
CGTCATT14650.017.18947826
CTCGATA13350.017.0594465
GTGTAGC49750.016.1265471
GCGCGAT6650.016.08216513
CATGGGC188750.015.8667174
ACGTGCG7200.015.8119679
Produced by FastQC (version 0.11.5)