FastQCFastQC Report
Thu 26 May 2016
ERR488957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138542
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502280.3317888869350826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357420.23609935487842884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261350.1726388181900212No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153070.10111277558961754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG9850.027.6559824
ATAACGC9950.027.452033
CGTCACT17050.026.9832861
TGCGACG10900.024.66607922
CTCGATA11900.022.33551865
TAACGCA13050.021.9909424
CAATGCG12600.021.88626719
TACCTGG131750.021.5224482
TAGCCCT129400.021.3762744
ATGCGAC15050.021.0703521
GAGTACT798650.020.94540412
GTGTAGC88950.020.805371
AGTACTT808450.020.7362213
CGATAAT13350.020.16960567
GTACCTG151700.019.883761
GTACTTT851100.019.58268414
GCTCGAT13300.019.46351864
TCGTCTA14000.019.22304254
CGCACTA13700.018.8881358
GTACATG994000.018.8199251
Produced by FastQC (version 0.11.5)