FastQCFastQC Report
Thu 26 May 2016
ERR488957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138542
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460360.30409797720282444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365810.24164150021844905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280230.18511029661905354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158990.10502332391058532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG9500.025.78256224
CGTCACT17800.024.111251
CTCGATA12150.023.00027865
TGCGACG10850.022.57459622
CGCGATA4600.022.49938214
CGATAAT12600.022.45265467
GCTCGAT12900.022.19794364
TACCTGG142000.022.0367532
ATAACGC9050.021.7298323
GTACCTG163900.019.9347651
ACCGTCG7450.019.9114823
TCGTCTA13900.019.6066654
ACGTCGT3350.019.56591423
GTACCCG11850.019.2771851
ACCTGGG155200.019.2733613
GTGTAGC91450.018.8100831
GAGTACT810950.018.73892412
AGTACTT822050.018.4823113
TAGCCCT136200.018.3392494
CAATGCG13350.018.0893319
Produced by FastQC (version 0.11.5)