FastQCFastQC Report
Thu 26 May 2016
ERR488956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22529486
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477700.21203324390090392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334290.14837888445391076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243420.10804507479664649No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA232140.10303830278240703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG295650.026.6874392
GTACCTG307150.026.215751
ACCTGGG343350.021.9792963
GAGTACT903450.020.640912
GTACATG1155650.020.5527781
AGTACTT918650.020.20731513
TACATGG1174350.019.8529362
TACCCGG35000.019.6586722
GTACTTT956600.019.3132614
ACATGGG1201400.018.8070853
AGAGTAC1626150.018.68525911
GTACCCG39600.018.3423061
TACTTTT1012250.018.22664615
ACTTTTT1066050.017.59565516
GTATCAA1963800.017.5654141
CATGGGG657250.016.6736094
TATCACG13300.016.637862
CATGGGT196250.016.2941994
TATCAAC2242000.015.304542
CCTGGGG277600.015.2427364
Produced by FastQC (version 0.11.5)