FastQCFastQC Report
Thu 26 May 2016
ERR488956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22529486
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA830100.36845048306916545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453510.20129620356185668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346430.15376737844795926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261230.11595027068083133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG294800.024.552962
GTACCTG309700.023.9816191
ACCTGGG338600.020.4904693
GAGTACT925400.019.64240612
AGTACTT940050.019.21578413
GTACATG1164650.018.8757861
GTACTTT970950.018.51897814
TACATGG1189400.018.2916092
TACCCGG32850.017.9591832
AGAGTAC1686800.017.5312411
ACATGGG1204650.017.5302563
TACTTTT1029650.017.45316515
ACTTTTT1076050.016.84164216
GTATCAA1978900.016.556881
CATGGGT208950.016.4615944
GTACCCG38550.016.2496551
TATAACG17600.015.4860352
CATGGGG647650.015.2936774
TATCACG13000.015.1271592
GGTATCA1533750.014.87033751
Produced by FastQC (version 0.11.5)