FastQCFastQC Report
Thu 26 May 2016
ERR488955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26685429
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA396500.039.081841
GTATCAA762150.033.7227361
TCAACGC980600.025.8625954
ATCAACG980750.025.8610923
TATCAAC991650.025.725572
CAACGCA1000700.025.3022465
AACGCAG1046400.024.318236
ACGCAGA1207700.021.0314487
CGCAGAG1236500.020.545788
GAGTACT740400.020.47407512
AGAGTAC1235800.019.91326911
AGTACTT765650.019.7331213
GTACTTT776100.019.39068214
GTACATG934800.018.9909521
CAGAGTA1326200.018.87239610
GTACCTG264900.018.6615721
GCAGAGT1350650.018.60399
TACATGG951100.018.1960852
TACCTGG268150.017.7403162
TACTTTT856650.017.63885715
Produced by FastQC (version 0.11.5)