FastQCFastQC Report
Thu 26 May 2016
ERR488955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26685429
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA399250.038.68221
GTATCAA769250.033.3814431
ATCAACG1000800.025.352733
TCAACGC1002650.025.28214
TATCAAC1018150.025.0630262
CAACGCA1027650.024.6771285
AACGCAG1069000.023.7935966
ACGCAGA1236600.020.5122727
GAGTACT766000.020.01431812
CGCAGAG1269950.019.9580888
AGAGTAC1267450.019.45845411
AGTACTT792350.019.28342213
GTACTTT800850.018.91936914
CAGAGTA1361450.018.4268110
GTACATG930200.018.2425631
GCAGAGT1396600.018.0667179
GTACCTG261250.017.9398021
GTGGTAT192900.017.644021
TACATGG951500.017.5031782
TACTTTT876800.017.34671215
Produced by FastQC (version 0.11.5)