FastQCFastQC Report
Thu 26 May 2016
ERR488954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13795023
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1948361.4123644447711323No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA562100.40746579400411287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183580.13307698000938453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG190650.030.3723321
TACCTGG188800.029.9756242
TATAACG8900.026.0259362
ACCTGGG222850.024.8733863
GAGTACG30850.023.9686371
TACGCAG30450.022.4692024
GTACCCG21550.020.6196651
CCTGGGG168700.020.2987064
GAGTACT453650.019.98231712
GTACATG575000.019.7966861
AGTACTT464150.019.53856313
TACATGG581100.019.2045542
ACGCAGT35050.018.7292185
GTACTTT490000.018.46591814
AGAGTAC792500.018.37716911
ACATGGG593600.018.0996423
TACCCGG23400.018.0245952
TACTTTT521100.017.3852315
ATAACGC15950.017.116293
ACTTTTT547200.016.74945416
Produced by FastQC (version 0.11.5)