FastQCFastQC Report
Thu 26 May 2016
ERR488954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13795023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA575110.4168967315241156No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA454950.32979285355305316No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG429830.3115833877188896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188220.13644051191505807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138410.10033328686729989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG184900.030.4731031
TACCTGG184800.030.0765062
GAGTACG33950.025.8777451
ACCTGGG219900.024.2245583
TATAACG9650.023.9541782
TACGCAG34200.022.6973954
ACGCAGT37050.020.5789725
GAGTACT467550.019.56742112
TACCCGG21300.019.27532
GTACCCG21700.019.1273141
CCTGGGG160550.019.0389524
AGTACTT478400.019.03003313
TACACGG22200.018.649282
GTACATG567750.018.355851
AGAGTAC814750.018.20674111
GTACTTT499350.018.08655514
TACATGG571300.017.923942
ATAACGC15250.017.64653
TATCACG10000.017.2505842
TAACGCA13450.017.1858944
Produced by FastQC (version 0.11.5)