FastQCFastQC Report
Thu 26 May 2016
ERR488953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13476497
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144160.10697141846282457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA343350.035.4927331
GTATCAA604450.030.3133141
TACCTGG208600.028.7761822
GTACCTG212400.028.7607381
ACCTGGG229950.025.2137363
TATCAAC753000.024.0988672
TCAACGC763500.023.746844
ATCAACG767250.023.6570153
CAACGCA776100.023.3655665
AACGCAG804900.022.7442866
GAGTACT454100.020.8699912
AGTACTT461750.020.44393313
CCTGGGG183500.019.858974
ACGCAGA919650.019.8312857
GTACTTT479800.019.56492814
CGCAGAG937250.019.4228348
AGAGTAC888800.019.3456811
TATCACG10900.019.033672
CAGAGTA966600.018.23786210
TACTTTT518400.018.11071415
Produced by FastQC (version 0.11.5)