FastQCFastQC Report
Thu 26 May 2016
ERR488953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13476497
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA340750.036.8610531
GTATCAA591900.029.8688981
TACCTGG209950.027.7880422
GTACCTG217050.027.2998731
ACCTGGG232550.024.1530233
TATCAAC756400.023.2575382
ATCAACG764100.022.9243
TCAACGC764000.022.917294
CAACGCA779700.022.4998255
AACGCAG807850.021.8610046
GAGTACT463600.020.19565612
AGTACTT475200.019.66709713
CCTGGGG181450.019.2797574
ACGCAGA928550.018.9627697
GTACTTT487100.018.93873414
CGCAGAG947350.018.5827458
AGAGTAC914650.018.50016611
ATAACGC15100.018.278753
TACTTTT522450.017.67711315
CAGAGTA993200.017.46071610
Produced by FastQC (version 0.11.5)