FastQCFastQC Report
Thu 26 May 2016
ERR488952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16076782
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248470.154552073916285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185660.115483310030577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA427800.032.8253941
TACCTGG229550.031.6613452
GTACCTG237200.031.1681291
TATAACG11450.029.5933782
GTATCAA733750.028.771021
ACCTGGG267750.026.3143463
TATCAAC904550.023.1487332
ATCAACG914500.022.8863543
TCAACGC916600.022.8548934
CAACGCA933000.022.4540335
AACGCAG963050.021.8863746
TAACGCA16250.021.0604784
TATCACG11100.020.870162
GAGTACT609450.020.51426912
AGAGTAC989950.020.00696411
AGTACTT625350.019.97733313
CCTGGGG202800.019.6538564
ACGCAGA1080500.019.3923267
ATAACGC17950.019.0672763
CGCAGAG1098850.019.0354848
Produced by FastQC (version 0.11.5)