FastQCFastQC Report
Thu 26 May 2016
ERR488952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR488952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16076782
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243910.15171568539027275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192520.11975033312014804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA422950.033.4732861
TACCTGG244000.031.4452422
GTACCTG252600.030.9911331
GTATCAA716050.028.359261
ACCTGGG274700.027.0518553
TATAACG13250.026.0371912
TATCAAC904150.022.3712082
ATCAACG909100.022.150733
TCAACGC912300.022.1032874
TAACGCA17150.021.9266554
CAACGCA931500.021.6737565
CCTGGGG205800.021.2896424
AACGCAG965350.021.0349466
ATAACGC19600.019.8898933
GAGTACT627300.019.7262512
AGTACTT645800.019.16667713
AGAGTAC1023050.018.94099211
ACGCAGA1090100.018.6269767
CGCAGAG1111800.018.2356598
GTACTTT678250.018.07165114
Produced by FastQC (version 0.11.5)