FastQCFastQC Report
Thu 2 Jun 2016
DRR025057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5042380
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163050.32335920735843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131220.2602342544592038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.16436682677624456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93000.058.1384121
GTATCAA179850.044.258311
ATCAACG233200.033.6231353
TCAACGC235750.033.277744
TATCAAC240350.032.9593162
GTGGTAT39600.031.9378281
CAACGCA248450.031.6337555
TGGTATC38650.030.5313932
AACGCAG259000.030.468926
ACGCAGA288300.027.0128927
CGCAGAG289250.026.761158
GTACATG215800.026.7326131
TACATGG214350.025.9687252
ACATGGG218100.024.7432613
GCAGAGT308500.024.6032969
CATGGGG118850.022.1481784
AGAGTAC289450.021.08368510-11
GAGTACT192800.020.95106912-13
CAGAGTA302200.020.76957110-11
AGTACTT199650.020.30285612-13
Produced by FastQC (version 0.11.5)