FastQCFastQC Report
Thu 2 Jun 2016
DRR025057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5042380
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182140.3612183135741456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151190.29983856829513045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105670.20956373775875675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95550.058.63491
GTATCAA189400.045.0290681
ATCAACG250150.033.658143
TATCAAC257100.032.9680252
TCAACGC255500.032.909054
GTGGTAT40600.031.6309091
CAACGCA266050.031.5687375
TGGTATC40750.030.3408282
AACGCAG277600.030.3399246
GTACATG223650.026.5649811
ACGCAGA317000.026.3447427
CGCAGAG317200.026.2831638
TATAACG4750.025.7323272
TACATGG223950.025.3999942
GCAGAGT338650.024.0910579
ACATGGG228500.024.071033
AGAGTAC320150.020.9839810-11
GAGTACT215700.020.62185112-13
CAGAGTA329950.020.42481810-11
AGTACTT222400.020.29643412-13
Produced by FastQC (version 0.11.5)