FastQCFastQC Report
Thu 2 Jun 2016
DRR025056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699344
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92770.250774191316082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76610.20709077068799225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.12237304776198157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59300.058.3465921
GTATCAA119950.041.503821
ATCAACG141850.034.9280473
TCAACGC142850.034.5834124
CAACGCA150750.032.864185
TATCAAC152650.032.520232
AACGCAG159350.031.1465766
GTGGTAT25650.028.957571
CGCAGAG175950.027.9401728
ACGCAGA178700.027.589487
TGGTATC26000.027.482532
GCAGAGT189250.025.1815769
TCTGTCG9550.024.6066978
GTACATG100850.023.9129871
TACATGG99250.022.9719432
GTCTCGC11750.022.0047871
ACATGGG103600.021.3709663
AGAGTAC179700.021.21062910-11
GAGTACT138150.021.14307412-13
CAGAGTA189800.021.0847910-11
Produced by FastQC (version 0.11.5)