FastQCFastQC Report
Thu 2 Jun 2016
DRR025056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699344
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96570.26104628280041003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86400.23355492216998475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56940.15391918134674687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.058.6646041
GTATCAA116100.042.742511
ATCAACG147650.033.174463
TCAACGC149150.032.80314
TATCAAC155450.031.7215482
CAACGCA157050.031.2128775
AACGCAG164450.029.8655016
TGGTATC24400.027.9349422
GTGGTAT26650.027.1808641
GTACCGG1755.515176E-926.8782961
CGCAGAG184550.026.1027038
ACGCAGA186650.025.9604517
TCTGTCG11900.024.8810838
GTACATG99450.024.8310051
ACAACGC6750.024.3743973
TACATGG97850.023.828162
GCAGAGT200300.023.4636049
ACATGGG100700.022.2668383
AGAGTAC188250.021.2831110-11
TACCTGG25950.021.1942542
Produced by FastQC (version 0.11.5)