FastQCFastQC Report
Thu 2 Jun 2016
DRR025055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105760
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181800.44279256459218264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145580.3545750360469194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.2242459374147539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.059.0269781
GTATCAA187850.043.122741
ATCAACG235950.034.050023
TCAACGC238800.033.7211534
TATCAAC242900.032.9999542
CAACGCA250250.032.159485
AACGCAG261050.030.9512846
GTGGTAT39500.029.8750691
TGGTATC38600.029.7179552
ACGCAGA288300.027.6817237
CGCAGAG291100.027.2691548
GTACATG192400.025.9019871
TACATGG189100.025.4580352
GCAGAGT303650.025.305999
ACATGGG197550.023.5826113
TACCTGG27050.021.898772
CAGAGTA297800.021.84189410-11
GAGTACT199900.021.41762412-13
AGAGTAC286650.020.80610-11
GTACTTT204650.020.65654814-15
Produced by FastQC (version 0.11.5)