FastQCFastQC Report
Thu 2 Jun 2016
DRR025055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105760
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197670.4814455788940415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162360.3954444487744047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113800.27717158333658076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.1065332605900004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98250.058.1132851
GTATCAA183500.043.00761
ATCAACG246100.031.6312083
TCAACGC248650.031.2821984
TATCAAC253250.030.86962
CAACGCA257100.030.363745
GTGGTAT40250.029.0952951
AACGCAG269850.028.9809996
TGGTATC38900.028.8826162
GTACATG195350.026.8201431
TACATGG192000.026.3940352
TATAACG4650.026.285042
ACGCAGA305500.025.3670967
CGCAGAG306950.025.0635368
ACATGGG200300.024.477693
GCAGAGT320500.023.5786699
CATGGGG97750.021.492084
CATGGGA85900.020.9552754
AGAGTAC304350.020.75330410-11
ATAACGC6350.020.7277153
Produced by FastQC (version 0.11.5)