FastQCFastQC Report
Thu 2 Jun 2016
DRR025054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411974
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110030.32248194153882764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92220.27028341951023077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.1638640857169486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62850.054.3834951
GTATCAA120200.041.6565321
ATCAACG144100.034.3860863
TCAACGC146600.033.702014
CAACGCA151550.032.569725
TATCAAC152850.032.4493452
AACGCAG159350.031.1188666
GTGGTAT28350.029.685061
ACGCAGA179200.027.3292457
TGGTATC29550.027.0464522
CGCAGAG180700.026.9455348
TACCTGG31050.024.8313962
GCAGAGT192000.024.821159
GTACATG108750.024.5127331
GTACCTG34550.023.5416031
TACATGG107600.023.2443962
ACAACGC6350.021.4699173
ACATGGG112150.021.4623283
CAGAGTA187750.021.19006210-11
AGAGTAC181350.020.79757310-11
Produced by FastQC (version 0.11.5)